Abnormalities of the P53, MDM2, BCL2 and BAX genes in acute leukemias.

摘要: Abnormalities of the P53 network have been implicated in pathogenesis acute lymphoblastic leukemia (ALL) and myeloblastic (AML). The purpose this study was to define gene mutations, detect MDM2 amplification estimate mRNA expression P53, MDM2, BCL2 BAX genes patients with ALL AML. Twenty-five 65 AML, both recently diagnosed, were included into study. Exons 5-8 flanking intronic sequence amplified by polymerase chain reaction (PCR) method subjected mutation screening single-strand conformation polymorphism analysis (SSCP). Mutation found one patient 25 five Sequence subsequently performed. One four missense mutations silent nucleotide substitution AML identified. Amplification detected multiplex-PCR only sample from ALL, but not observed any case To gain further insight role evolution leukemias, expressions a portion samples analyzed using multiplex RT-PCR. Although low frequency molecular disturbances study, there high percentage cases increased level A overexpression relatively leukemias indicate that altered transcription these may be involved leukemogenesis.