Single-cell analysis reveals the relevance of foot-and-mouth disease virus persistence to emopamil-binding protein gene expression in host cells.

摘要: Foot-and-mouth disease virus (FMDV) infects host cells in either an acute or persistent manner. In this study, we examined the relevance of establishment FMDV persistence to expression emopamil-binding protein (EBP) gene 231 individual persistently infected baby hamster kidney (BHK-21) after passages 28, 38, and 68 (PI28, PI38, PI68). At PI28, stage at which infection FDMV becomes unstable, percentage carrying was 66.7%, while 80.2% were EBP positive. Additionally, 55.6% EBP-positive upregulated approximately 149.9% compared uninfected BHK-21 cells. This highest level among all cell measured. Interestingly, a parallel experiment, average whole population PI28 only slightly higher (108.2%) than that 98.7% positive for 3D (an RNA-dependent RNA polymerase enzyme gene), its maximum observed passage. The 78.2% total cells, however, reduced significantly. PI68, 95.8% positive, both genes lowest levels passages. Our studies using single yielded data are otherwise inaccessible population. These results suggest by is dynamic process from continuous adaptation coevolution viruses reach equilibrium.