Thymus-dependent network responses to a monoclonal cross-reactive antiidiotypic antibody.

摘要: BALB/c mice were inoculated i.p. with a cross-reactive anti-Idiotypic mAb (designated FD5-1) in the absence of Ag or adjuvants. Injection unmodified FD5-1 resulted induction serum antibodies reactive both (Ab3) and hapten DNP (Ab1'). Endpoint titers Ab3 response showed an increase IgM, which was dose-responsive to number injections amount antibody injected. The IgM found be thymus dependent idiotypically specific for FD5-1. Athymic injected unable produce response, whereas their euthymic littermates produced strong responses. Serum responses Ab1' detectable only isotype; no IgG observed. Indeed, recognized by appeared suppressed modulated DNP, (4-hydroxy-3-nitrophenyl)acetyl (NP), 4-ethoxymethylene-2-phenyloxazol-5-one (OX), phosphorylcholine (PC), alpha 1,3-dextran (DEX) thymus-dependent manner. injection induced against 4-ethoxymethylene-2-phenyloxazol-5-one, DEX but decreased binding PC. No any aforementioned molecules when same sera probed IgG. specificity from FD5-1-injected evaluated antigenic inhibition. Binding DNP-BSA inhibitable DNP-lysine, equivalent concentrations lysine alone had inhibitory effect. normal PC-BSA demonstrated inhibition free PC, DEX-coated plates shown DEX. We have described vivo network perturbation adult anti-Id Our findings show that can induce Ag-specific In two systems where functions as anti-anti-anti-Id (PC DEX), also DEX-specific induced.