Covalent immobilization of lipase onto hydrophobic group incorporated poly(2-hydroxyethyl methacrylate) based hydrophilic membrane matrix

摘要: Abstract In this study, a hydrophobic group containing monomer, 2-methacrylamidophenyalanine (MAPA) was prepared by using methacrylochloride and phenylalanine. Then, poly(2-hydroxyethyl methacrylate-co-methacrylamido-phenlyalanine) (pHEMA-MAPA) membranes were UV-initiated photopolymerization of HEMA MAPA in the presence an initiator α-α ′ -azobisisobutyronitrile (AIBN). The lipase immobilized onto these covalent bonding through carbodiimide activation. amount enzyme loading on increased as ratio membrane structure. Immobilization improved pH stability well its temperature stability. Thermal found to increase with immobilization at 60 ° C thermal constants 1.1×10 −1 min for free 1.2×10 −2 enzyme. activity be quite stable repeated experiments.