The extracellular matrix protein agrin is essential for epicardial epithelial-to-mesenchymal transition during heart development.
作者： Eldad Tzahor , Sophia Malandraki-Miller , Sophia Malandraki-Miller , Elad Bassat , Paul R Riley
DOI: 10.1242/DEV.197525
关键词:
摘要: During heart development, epicardial cells residing within the outer layer undergo epithelial-mesenchymal transition (EMT) and migrate into underlying myocardium to support organ growth morphogenesis. Disruption of EMT results in embryonic lethality, yet its regulation is poorly understood. Here, we report mesothelial mouse at ultra-high resolution using scanning electron microscopy combined with immunofluorescence analyses. We identified morphologically active regions that associated key components extracellular matrix, including basement membrane-associated proteoglycan agrin. Deletion agrin resulted impaired compromised development epicardium, accompanied by downregulation Wilms' tumor 1. Agrin enhanced human stem cell-derived epicardial-like decreasing β-catenin promoting pFAK localization focal adhesions, promoted aggregation dystroglycan Golgi apparatus murine cells. Loss dispersal vivo, disrupting membrane integrity impairing EMT. Our provide new insights role matrix implicate as a crucial regulator
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