Tuning three-dimensional collagen matrix stiffness independently of collagen concentration modulates endothelial cell behavior

摘要: Numerous studies have described the effects of matrix stiffening on cell behavior using two-dimensional synthetic surfaces; however, less is known about cells embedded in three-dimensional vivo-like matrices. A primary limitation investigating stiffness three dimensions lack materials that can be tuned to control independently density. Here, we use collagen-based scaffolds where mechanical properties are non-enzymatic glycation collagen solution, prior polymerization. Collagen solutions glycated polymerization result gels with a threefold increase compressive modulus without significant changes architecture. Using these scaffolds, show endothelial spreading increases stiffness, as does number and length angiogenic sprouts overall spheroid outgrowth. Differences sprout maintained even when receptor for advanced end products inhibited. Our results demonstrate ability de-couple from density structure gels, increased sprouting