Effect of ovary holding temperature and time on equine granulosa cell apoptosis, oocyte chromatin configuration and cumulus morphology

摘要: Abstract The effects of ovary holding time and temperature on granulosa cell apoptosis, oocyte chromatin configuration cumulus morphology were investigated through a series experiments. Three experiments performed to determine the effect apoptosis. Ovaries held (1) at 20, 30 or 35–37 °C for up 2 h, (2) 30 °C 0–1, 1–2, 2–3, 3–4, 4–6 6–10 h, (3) cells 0, 1, 2, 3, 5, 12 24 h in M199 with Hank’s salts room (suboptimal incubation). Granulosa DNA was analysed by ethidium bromide staining 3′-end labelling. Two configuration. 3 h 20–37 °C 4–6, 6–8 8–12 h. oocytes stained Hoechst stain 33258 evaluated. oophorus morphology. 20–30 2 h 0–2, 2–4, 6–10 h 35–37 °C. complex (COC) retrieved There no difference proportion follicles non-apoptotic two groups below body (20 30 °C), but more had apoptotic when ovaries (P