Differentially expressed genes in hemocytes of red swamp crayfish Procambarus clarkii following lipopolysaccharide challenge

摘要: Abstract Procambarus clarkii is an important aquatic organism in China, but its aquaculture has suffered great economic losses due to pathogen infections. To better understand the P. immune response, we used RNA sequencing (RNA-seq) examine expression responses of hemocyte transcriptome lipopolysaccharide (LPS). Through assembly and annotation, a total 53,910 unigenes were identified, with average length 1246 bp. 589 differentially expressed genes (DEGs) obtained by injection LPS 310 upregulated 279 downregulated genes. Kyoto Encyclopedia Genes Genomes enrichment analysis identified several response pathways. Additionally, lot DEGs do Ras signaling pathway, lysosome, Rap1 mitogen-activated protein kinase pathway after challenge. Results Real-time quantitative reverse transcription PCR revealed that 11 randomly selected stimulation compared phosphate-buffered saline stimulation, this result also validated RNA-seq data. Our data further enrich databases provide basis for system defense mechanisms against