Influence of injection site, microvascular pressureand ultrasound variables on microbubble-mediated delivery of microspheres to muscle

摘要: Abstract Objectives Our objective was to test the hypothesis that ultrasound pulsing interval (PI), microbubble injection site and microvascular pressure significantly influence transport of 100-nm microspheres muscle through extravasation sites created by destruction microbubbles with ultrasound. Background Microbubbles show promise as targeted drug gene delivery agents; however, designing optimal microbubble-based therapies will require an understanding factors microbubble-delivered, gene-bearing vehicles tissue. Methods Ultrasound at 1 MHz, a peak negative amplitude 0.75 MPa, applied in exteriorized rat spinotrapezius muscle. PIs 1, 3, 5 10 s, arterial microsphere times or 40 s versus venous were tested. Results Extravasation point creation greatest when PI s. No significant differences observed injection, but trend toward increased may exist. Decreasing time from pressure, which, turn, substantially enhanced tissue, without concomitant increase number points. Conclusions The points These be important optimizing contrast ultrasound-based therapies.