Process for desilylation of oligonucleotides
作者： Muthiah Manoharan , Rajendra K. Pandey , Michael E. Jung , Gang Wang , Kallanthottathil G. Rajeev
DOI:
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摘要: The present invention relates to processes and reagents for oligonucleotide synthesis purification. One aspect of the compounds useful activating phosphoramidites in synthesis. Another a method preparing oligonucleotides via phosphoramidite using an activator invention. sulfur-transfer agents. In preferred embodiment, agent is 3-amino-1,2,4-dithiazolidine-5-one. phosphorothioate by treating phosphite with reagent that scavenge acrylonitrile produced during deprotection phosphate groups bearing ethylnitrile protecting groups. scavenger polymer-bound thiol. agents used oxidize phosphate. oxidizing sodium chlorite, chloroamine, or pyridine-N-oxide. methods purifying annealing first single-stranded second form double-stranded oligonucleotide; subjecting chromatographic purification high-performance liquid chromatography.
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