作者: Jacob N. Wohlstadter
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摘要: The present application provides a method for generating biocatalyst, comprising: selecting sequence encoding functional catalytic domain of an enzyme; inserting said into the genetic cell such that said may undergo immunoglobulin superfamily diversification; permitting diversification to occur generate a plurality putative biocatalysts; and identifying biocatalyst having desired activity within plurality biocatalysts. Also provided are biocatalysts obtainable by method.