Ebastine inhibits T cell migration, production of Th2-type cytokines and proinflammatory cytokines.

摘要: Summary Background  Cytokine imbalance and cellular migration to inflammatory sites are critical components of allergic diseases. Redirecting cytokine inhibiting cell therefore represent important therapeutic strategies for the treatment these disorders. Objectives  To study in vitro effect ebastine, a novel non-sedating H1 receptor antagonist, on secretion activated T cells, as well production pro-inflammatory cytokines by macrophages. Methods  Peripheral cells obtained from healthy volunteers were cultured wells coated with combination anti-CD3 monoclonal antibody (mAb) anti-CD26 mAb, mAb anti-CD28 or PMA, presence absence ebastine. proliferation measured [3H]thymidine incorporation assay ELISA, respectively. In addition, transendothelial macrophages examined. Results  Ebastine inhibited IL-4, IL-5, IL-6, TNF-α under each co-stimulatory condition tested, whereas it exhibited no IL-2 IFN-γ. such IL-6 ebastine. Conclusions  These results indicate that ebastine has specific inhibitory Th2-type production. Moreover, macrophages, suggesting might be useful cell-mediated disorders, including asthma, atopic dermatitis, autoimmune