Approach to produce hiv-1 gp140 envelope protein trimers
作者： Venigalla B. Rao , Wadad Alsalmi
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摘要: An approach of producing recombinant trimers that mimic native HIV-1 envelope is developed. A protein forming the encompasses a gp140 fused to tag through linker at C-terminus gp140. The sufficiently long so accessible for binding by molecule bound on solid matrix. After expressed in cell, secreted into culture medium and assembles therein. may be directly purified from medium. Cleaved uncleaved different clade viruses are produced.
lens.org 参考文章(102)
Craig B. Wilen, John C. Tilton, Robert W. Doms, Molecular mechanisms of HIV entry. Advances in Experimental Medicine and Biology. ,vol. 726, pp. 223- 242 ,(2012) , 10.1007/978-1-4614-0980-9_10
Sanjay Phogat, Maria J. Chiuchiolo, Ross W. B. Lindsay, Aaron J. Wilson, Maoli Yuan, Christopher L. Parks, Svetlana Rabinovich, Recombinant viral vectors ,(2013)
Michael Farzan, Peter D. Kwong, Xinzhen Yang, Richard Wyatt, Joseph G. Sodroski, Stabilized soluble glycoprotein trimers ,(2000)
Yusen Zhou, Lanying Du, Shibo Jiang, Influenza hemagglutinin-specific monoclonal antibodies for preventing and treating influenza virus infection ,(2011)
Peter Burkhard, Self-assembling peptide nanoparticles useful as vaccines ,(2009)
A Trkola, M Purtscher, T Muster, C Ballaun, A Buchacher, N Sullivan, K Srinivasan, J Sodroski, J P Moore, H Katinger, Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. Journal of Virology. ,vol. 70, pp. 1100- 1108 ,(1996) , 10.1128/JVI.70.2.1100-1108.1996
G. D. Cross, M. S. Kennedy, S. P. Cort, J. K. A. Nicholson, J. S. Mcdougal, A. C. Mawle, Binding of the human retrovirus HTLV-III/LAV/ARV/HIV to the CD4 (T4) molecule: conformation dependence, epitope mapping, antibody inhibition, and potential for idiotypic mimicry. Journal of Immunology. ,vol. 137, pp. 2937- 2944 ,(1986)
P Roben, J P Moore, M Thali, J Sodroski, C F Barbas, D R Burton, Recognition properties of a panel of human recombinant Fab fragments to the CD4 binding site of gp120 that show differing abilities to neutralize human immunodeficiency virus type 1. Journal of Virology. ,vol. 68, pp. 4821- 4828 ,(1994) , 10.1128/JVI.68.8.4821-4828.1994
Wadad AlSalmi, Marthandan Mahalingam, Neeti Ananthaswamy, Christopher Hamlin, Dalia Flores, Guofen Gao, Venigalla B. Rao, A New Approach to Produce HIV-1 Envelope Trimers: BOTH CLEAVAGE AND PROPER GLYCOSYLATION ARE ESSENTIAL TO GENERATE AUTHENTIC TRIMERS. Journal of Biological Chemistry. ,vol. 290, pp. 19780- 19795 ,(2015) , 10.1074/JBC.M115.656611
Richard Wyatt, Viktoriya Dubrovskaya, Francisco Javier Guenaga, Soluble hiv-1 envelope glycoprotein trimers ,(2016)