Pyrosequencing-Based Comparative Genome Analysis of Vibrio vulnificus Environmental Isolates

摘要: Between 1996 and 2006, the US Centers for Disease Control reported that only category of food-borne infections increasing in frequency were those caused by members genus Vibrio. The Gram-negative bacterium Vibrio vulnificus is a ubiquitous inhabitant estuarine waters, number one cause seafood-related deaths US. Many V. isolates have been studied, it has shown two genetically distinct subtypes, distinguished 16S rDNA other gene polymorphisms, are associated predominantly with either environmental or clinical isolation. While local genetic differences between subtypes probed, genomes so far completely sequenced. In order to better understand as an agent disease identify molecular components its virulence mechanisms, we completed whole genome shotgun sequencing three diverse genotypes using pyrosequencing approach. strain JY1305 was sequenced depth 33×, strains E64MW JY1701 lesser depth, covering approximately 99.9% each genome. We performed comparative analysis these sequences against previously published isolates. find dynamic, 1.27% genes C-genotype not found E- genotype genomes. identified key differentiate genotypes. 167 be specifically 278 Genes specific include sialic acid catabolism, mannitol fermentation, component Type IV secretory pathway VirB4, well several potential significance human virulence. included may implications balance self-preservation under stress nutritional competence.