A possible role for proteases and deaminases in the development of the symptoms of bacterial speck disease in tomato caused by Pseudomonas syringae pv. tomato

摘要: Pseudomonas syringae pv. tomato produces constitutive proteases during the logarithmic phase of growith in culture, which have optimum activity at pH 7·0 and 30°C. The addition proteina-ceous compounds to growth medium caused only a slight increase specific enzyme preparation. Proteolytic was higher diseased tissue susceptible plants than resistant plants, reaching maximum levels later stages infection. Total proteolytic appeared be due four proteases, two originating from pathogen, one host produced interaction between pathogen its host. A correlation disease severity infected demonstrated, with being greatest around developing necrotic zones. also varied age leaf time nitrogen content soluble proteins decreased development, asparagine free amino acids accumulating first 100 h after inoculation, but then decreasing. utilized five as source glutamine plant. Five different deaminases were detected but, five, asparaginase glutaminase suceptible cultivar. Liberation gaseous ammonia cultivar observed 72 inoculation. Similar changes occurred, on very limited scale, inoculated plants. increased production.