Gene amplification of 5-enol-pyruvylshikimate-3-phosphate synthase in glyphosate-resistant Kochia scoparia.

摘要: Field-evolved resistance to the herbicide glyphosate is due amplification of one two EPSPS alleles, increasing transcription and protein with no splice variants or effects on other pathway genes. The widely used inhibits shikimate enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Globally, intensive use for weed control has selected in 31 species. Populations suspected glyphosate-resistant Kochia scoparia were collected from fields located US central Great Plains. Glyphosate dose response verified nine populations. mechanism was investigated using targeted sequencing, quantitative PCR, immunoblotting, whole transcriptome de novo sequencing characterize sequence expression EPSPS. Sequence analysis showed mutation EPSPS Pro106 codon K. scoparia, whereas genomic copy number transcript abundance elevated three- ten-fold resistant individuals relative susceptible individuals. Glyphosate-resistant increased numbers had consistently lower accumulation leaf disks treated 100 μM levels higher gene compared glyphosate-susceptible RNA revealed seven nucleotide positions two different expressed alleles reads. However, at predominant sequences, suggesting that only amplified No alternatively spliced transcripts detected. Expression five genes chorismate unaffected expression. These results indicate a these