Multiplicación in vitro de Psidium guajava L. en sistemas de inmersión temporal
作者： Jorge Alberto Vilchez Perozo , Nilca Rosa Albany de Vilchez
DOI: 10.15446/REV.COLOMB.BIOTE.V16N2.42180
关键词:
摘要: Titulo en espanol: Multiplicacion in vitro de Psidium guajava L. sistemas inmersion temporal ingles: In multiplication of temporary immersion systems Resumen: La introduccion nuevos cultivares guayabo ( L.) amerita su propagacion masiva, lo cual solo puede ser satisfecho mediante la micropropagacion. Sin embargo micropropagacion convencional dejo economicamente eficiente, debido al uso agentes gelificantes y el elevado numero operaciones manuales, por esta razon se planteo investigacion, generar una metodologia que permita disminuir los costos produccion exclusion del gelificante medios cultivo, evaluando (SIT) multiplicacion guayabo. Para cual, evaluo efecto cultivo SIT, comparo SIT tipo BIT® RITA® tiempo(1 2 min) frecuencia(3 4 veces/dia) inmersion. Luego seis semanas evaluo: brotes(NB), nudos(NN), longitud brote(LB) coeficiente multiplicacion(CM). Con empleo logro valores superiores para NB(2,17), NN(3,5), LB(10,7 mm) CM(2,8). En comparacion entre RITA BIT, obtuvieron con NB(3,8), NN(3,8), LB(16,6 CM(10,4). Se determino min mayores NB(3,7), NN(13,4), LB(15,3 3-4 veces/dia mayor CM(9,4 10,4). concluye favorecio crecimiento proliferacion brotes Palabras clave: BIT®, guayabo, medio liquido, micropropagacion, RITA®. Abstract: The introduction new cultivars guava deserves its mass propagation, which can only be satisfied by micropropagation. However conventional micropropagation stopped being economically efficient due to the use gelling agents and high number manual operations. For this reason was considered research, generate a methodology reduce production costs culture media, assessing (TIS) guava. which, effect way evaluated TIS, type TIB® compared TIS time(1 frequency(3 times / day) immersions. After six weeks were evaluated: shoots number(NS), nodes number(NN), shoot length(SL) rate(MR). With higher values for NS(2.17), NN(3.5), SL(10.7 MC(2.8) achieved. When comparing TIB, obtained with NS(3.8), NN(3.8), SL(16.6 MC(10.4). It determined that highest NS(3.7), NN(13.4), SL(15.3 times/day achieved MC(9.4 10.4). We conclude favored growth proliferation guava. Keys words: guava, micropropagation, liquid RITA®, TIB®. Recibido: febrero19 2014 Aprobado: octubre 10 2014
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