Association of insulin-like growth factor binding protein-3 expression with melanoma progression

摘要: Previous studies from our laboratory have identified several endothelial cell-associated marker genes implicated in human melanoma metastasis via tumor vasculogenic mimicry. In this study, we used dual model systems composed of cell lines and clinical samples to validate the importance insulin-like growth factor binding protein-3 (IGFBP-3) as a involved disease progression. Gene expression analysis was done using microarray approach for both primary metastatic samples. The IGFBP-3 decreased small interfering RNA (siRNA) knockdown quantified with real-time quantitative reverse transcription-PCR analysis. protein 3 up-regulated by nearly 16-fold WM266-4 compared WM35 cells. A subsequent parallel freshly isolated tissue array confirmed previous findings. functional significance invasion further investigated siRNA gene approach, markedly reduced. Additionally, resulted significant reduction motility, migration, invasive capacity cells vitro. These results strongly suggest that may be vital proliferation necessary is an important biomarker melanoma.