Kinetic Analyses of Desulfurization of Dibenzothiophene by Rhodococcus erythropolis in Continuous Cultures

摘要: Rhodococcus erythropolis N1-36, a desulfurization strain, was grown in continuous culture at 10 different dilution rates with 50 (mu)M dibenzothiophene sulfone (DBTO(inf2)) as the growth-limiting nutrient. The steady-state biomass, concentrations of substrate and product (monohydroxybiphenyl), saturation constant (0.39 DBTO(inf2)), cell yield coefficient (9 mg biomass(middot)(mu)M(sup-1) DBTO(inf2)) were measured. Continuous cultures five temperatures allowed calculation activation energy (0.84 kcal(middot)mol(sup-1) [ca. 3.5 kJ(middot)mol(sup-1)]) near optimal temperature (30(deg)C) for growth. A washout technique used to calculate maximum specific growth rate (0.235 h(sup-1)), value equivalent minimum generation time 2.95 h.