Phosphorylation of CPI-17, an inhibitory phosphoprotein of smooth muscle myosin phosphatase, by Rho-kinase
作者： Mutsumi Koyama , Masaaki Ito , Jianhua Feng , Tetsuya Seko , Katsuya Shiraki
DOI: 10.1016/S0014-5793(00)01654-9
关键词:
摘要: Phosphorylation of CPI-17 by Rho-associated kinase (Rho-kinase) and its effect on myosin phosphatase (MP) activity were investigated. was phosphorylated Rho-kinase to 0.92 mol P/mol in vitro. The inhibitory phosphorylation site Thr38 (as reported previously) identified using a point mutant state-specific antibody. dramatically increased the MP activity. Thus, as substrate could be involved Ca2+ sensitization smooth muscle contraction downstream effector Rho-kinase.
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sci-hub.se 参考文章(27)
U. K. LAEMMLI, Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Nature. ,vol. 227, pp. 680- 685 ,(1970) , 10.1038/227680A0
H. Towbin, T. Staehelin, J. Gordon, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 4350- 4354 ,(1979) , 10.1073/PNAS.76.9.4350
Andrew P. Somlyo, Avril V. Somlyo, Signal transduction by G‐proteins, Rho‐kinase and protein phosphatase to smooth muscle and non‐muscle myosin II The Journal of Physiology. ,vol. 522, pp. 177- 185 ,(2000) , 10.1111/J.1469-7793.2000.T01-2-00177.X
Jianhua Feng, Masaaki Ito, Yasuko Kureishi, Kazuhito Ichikawa, Mutsuki Amano, Naoki Isaka, Katsuya Okawa, Akihiro Iwamatsu, Kozo Kaibuchi, David J. Hartshorne, Takeshi Nakano, Rho-associated Kinase of Chicken Gizzard Smooth Muscle Journal of Biological Chemistry. ,vol. 274, pp. 3744- 3752 ,(1999) , 10.1074/JBC.274.6.3744
L. Li, M. Eto, M. R. Lee, F. Morita, M. Yazawa, T. Kitazawa, Possible involvement of the novel CPI‐17 protein in protein kinase C signal transduction of rabbit arterial smooth muscle The Journal of Physiology. ,vol. 508, pp. 871- 881 ,(1998) , 10.1111/J.1469-7793.1998.871BP.X
K. Kaibuchi, S. Kuroda, M. Amano, Regulation of the cytoskeleton and cell adhesion by the Rho family GTPases in mammalian cells. Annual Review of Biochemistry. ,vol. 68, pp. 459- 486 ,(1999) , 10.1146/ANNUREV.BIOCHEM.68.1.459
Michael P. Walsh, Susan Hinkins, Renata Dabrowska, David J. Hartshorne, [30] Smooth muscle myosin light chain kinase Methods in Enzymology. ,vol. 99, pp. 279- 288 ,(1983) , 10.1016/0076-6879(83)99063-8
M Ikebe, D J Hartshorne, Effects of Ca2+ on the conformation and enzymatic activity of smooth muscle myosin. Journal of Biological Chemistry. ,vol. 260, pp. 13146- 13153 ,(1985) , 10.1016/S0021-9258(17)38850-6
DAVID J. HARTSHORNE, MASAAKI ITO, FERENC ERDO¨DI, Myosin light chain phosphatase: subunit composition, interactions and regulation. Journal of Muscle Research and Cell Motility. ,vol. 19, pp. 325- 341 ,(1998) , 10.1023/A:1005385302064
Masayoshi Uehata, Toshimasa Ishizaki, Hiroyuki Satoh, Takashi Ono, Toshio Kawahara, Tamami Morishita, Hiroki Tamakawa, Keiji Yamagami, Jun Inui, Midori Maekawa, Shuh Narumiya, Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension Nature. ,vol. 389, pp. 990- 994 ,(1997) , 10.1038/40187