Automated metabolic profiling of organic acids in human urine. I. Description of methods.
作者： S C Gates , N Dendramis , C C Sweeley
DOI: 10.1093/CLINCHEM/24.10.1674
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摘要: We describe a complete procedure for separation and mechanized analysis of organic acids in human urine. The acid fraction urine is separated by anion-exchange chromatography on diethylaminoethyl-Sephadex. Individual are identified measured use gas-chromatography/mass spectrometer/computer system that can clearly distinguish contributions from at least 150 substances single sample. discuss analytical recoveries, the sample process, stability stored samples before after processing, reproducibility extraction procedure.
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