Chondrogenic differentiation of human mesenchymal stem cells on photoreactive polymer-modified surfaces.
作者： Likun Guo , Naoki Kawazoe , Yujiang Fan , Yoshihiro Ito , Junzo Tanaka
DOI: 10.1016/J.BIOMATERIALS.2007.08.043
关键词:
摘要: Human mesenchymal stem cells (MSCs) were cultured on polystyrene surfaces modified with photoreactive azidophenyl-derivatives of three different chargeable polymers, poly(acrylic acid) (PAAc), polyallylamine (PAAm), and poly(ethylene glycol) (PEG). The MSCs adhered spread both a PAAm-modified surface PAAc-modified (control) surfaces. However, the more easily to surface. did not attach PEG-modified aggregated form pellets immediately after cell seeding. proliferated PAAc-, control culture time, formed monolayer, pellets. in that PAAm- 2 weeks had round morphology extracellular matrices positively stained by safranin O toluidine blue, while those spindle, fibroblast-like blue. contained significantly higher levels sulfated glycosaminoglycans than Type II collagen cartilage proteoglycan immunohistologically detected surfaces, but expressed high level cartilaginous genes encoding type aggrecan, express these genes. These results suggest supported adhesion proliferation also promoted chondrogenic differentiation MSCs. proliferation, differentiation. support adhesion, promote could be controlled chemistry.
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