Detection of microarray-hybridized oligonucleotides with magnetic beads.

摘要: The efficiency of hybridization analysis with oligonucleotide microarrays depends heavily on the method detection. Conventional methods based labeling nucleic acids fluorescent, chemiluminescent, enzyme, or radioactive reporters suffer from a number serious drawbacks which demand development new detection techniques. Here, we report two approaches for employing magnetic beads as active labels. In first streptavidin-coated are used to discover biotin-labeled DNA molecules hybridized arrayed probes. second bound surface and then complementary strands bead-array contacts. Using simple low-power microscope dark-field illumination pair primers model system evaluated sensitivity, speed, cost compared its performance techniques enzyme fluorescent It was shown that microarray-hybridized combines low high speed enhanced assay opening way routine assays do not require precise measurements concentration.