Rapid identification and quantitative validation of a caffeine-degrading pathway in Pseudomonas sp. CES

摘要: Understanding the genes and enzymes involved in caffeine metabolism can lead to applications such as production of methylxanthines environmental waste remediation. Pseudomonas sp. CES may provide insights into these applications, since this bacterium degrades thrives concentrations that are three times higher (9.0 g L–1) than maximum tolerable levels other reported bacteria. We took a novel approach toward identifying enzymatic pathways metabolize caffeine, which largely circumvented need for exhaustive isolation stepwise reconstitution their activities. Here we describe an optimized, rapid alternative strategy based on multiplexed LC-MS/MS assays show its application by discovering caffeine-degrading strain quantitative comparison proteomes from bacteria grown absence presence latter condition was found have highly induced capacity f...