Effects of U-74389G (21-Lazaroid) and Ascorbic Acid on Liver Recovery After Acute Ischemia and Reperfusion in Rats

摘要: BACKGROUND/AIM The free radical-scavenging effects of the lazaroid U-74389G have been shown in several experimental models to protect liver from ischemia/reperfusion (I/R), however, mechanism cytoprotection is not fully understood. Similar findings were observed when ascorbic acid was administered. This study investigates infusion and on cytokines structure a I/R rat model. MATERIALS AND METHODS Sixty male Wistars rats, weighting 220-290 g, used study. Six groups formed: Group 1 (control group): ischemia for 30 min reperfusion 60 min; group 2 120 3: min, intraportal injection acid, 4: administration, 5: 6: min. Tissue blood sampling took place upon completion each model's reperfusion. administered at 10 mg/kg animal body weight 100 mg/kg. Anesthesia induced with ketamine xylazine. Surgery performed through midline laparotomy. portal vein common hepatic artery isolated prepared occlusion. Blood samples wedge biopsies taken measure levels enzymes, microscopic analysis once RESULTS Histopathological evaluation revealed statistically significant reduction degree necrosis tissue treated compared control [groups 3, 5 (p=0.010) 4, 6 (p<0.0005)]. On other hand, malondialdehyde (MDA) significantly increased only between (p<0.0005). There no difference tumor factor-α groups. As alkaline phosphatase (ALP) gamma-glutamyl transferase (gGT) reduced 3 (ALP: p=0.027, gGT: p=0.002) 4 p=0.004, p=0.015) 2. CONCLUSION Based histological data some spite rise malondialdehyde, this model, administration (I/R) injury has potential attenuating damage.