Stimulation of DNA synthesis by tumour promoter and pure mitogenic factors
作者： PHILLIP DICKER , ENRIQUE ROZENGURT
DOI: 10.1038/276723A0
关键词:
摘要: 12-O-TETRADECANOYL PHORBOL-13-ACETATE (TPA), a potent tumour-promoting agent, stimulates the proliferation of various cell types, including fibroblasts, in culture (see ref. 1 for review). Recent studies have demonstrated that there is close correlation between potency phorbol esters promoting skin tumours and their ability to stimulate DNA synthesis cultured cells2. The growth-stimulating activity TPA completely dependent on presence serum nutrient medium. This requirement has been cultures mouse epidermal cells3 fibroblastic cells4,5. role cellular response remains unresolved. We report here can be replaced by polypeptide hormones like growth factor (EGF)6, insulin isolated from conditioned medium SV40 BHK cells (fibroblast-derived factor, FDGF)7. Low concentrations growth-factors quiescent human serum-free
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