RFLP Typing: A New Highly Polymorphic VNTR Locus and Chemiluminescent Detection

摘要: There are two basic DNA typing methodologies available to the forensic scientist for characterizing biological evidence. The first technology gain wide use in arena was of variable number tandem repeat (VNTR) loci by restriction fragment length polymorphism (RFLP) analysis. RFLP is well-defined, provides a high degree discrimination, and can be accomplished, at times, with less than 50 ng molecular weight genomic DNA. This methodology has been validated applications (Budowle Baechtel 1990). other strategy based on increasing copies target sequence amplification using polymerase chain reaction (PCR) (Saiki, et al. 1985). Since sequences interest increased dramatically PCR, simplified methods used determining polymorphisms sample. advantages PCR-based offers, compared currently employed approach, are: 1) augmented sensitivity specificity, 2) decreased assay time labor, 3) absence an isotopic label, 4) many degraded samples amplified PCR subsequently typed because alleles amenable much smaller size detected These qualities combine make PCRbased technologies extremely useful tools analyzing material found crime scenes.