Angiogenic properties of sustained release platelet-rich plasma: Characterization in-vitro and in the ischemic hind limb of the mouse

摘要: Background While single growth factor has limitation to induce optimal neovascularization, platelet-rich plasma (PRP) is an autologous reserver of various factors. However, little known about the mechanism PRP-related neovascularization.The objective this investigation was characterize angiogenic and content PRP determine, in vitro, its effect on endothelial cell proliferation. Additionally, experiment sought determine effectiveness different compositions (solution versus sustained release) perfusion neovascularization a murine model hind limb ischemia. Methods Different factors were measured by enzyme-linked immunosorbent assay (ELISA). In vivo study, we used gelatin hydrogel as release carrier for PRP. We induced ischemia excising right femoral artery wild type C57BL6 mice. After surgery, mice randomly assigned four experimental groups; control (C), 100 μL form platelet-poor (PPP), solution (PRP-sol), (PRP-sr); each formulation administered via intramuscular injection ischemic limb. Endpoint evaluations blood laser Doppler image, vascular density anti Von Willebrand (vWF), mature vessel smooth muscle actin (SMA) antibody. Green fluorescent protein (GFP+) transgenic generated transplantation bone marrow derived mononuclear cells mice, finally CD34+ site detected staining with anti-CD34 Results vitro study showed that containing induces proliferation capillary tube formation. demonstrated increased tissue imaging (LDPI) (57 ± 12, 56 9, 72 7, 98 4 groups C, PPP, PRP-sol, PRP-sr, respectively; P 2 PRP-sr respectively, Conclusion Sustained potent restores presumably stimulating angiogenesis, arteriogenesis, well vasculogenesis mouse