Cell inactivation and membrane damage after long-term treatments at sub-zero temperature in the supercooled and frozen states.

作者: Marwen Moussa , Frédéric Dumont , Jean-Marie Perrier-Cornet , Patrick Gervais

DOI: 10.1002/BIT.21981

关键词:

摘要: The survival of cells subjected to cooling at sub-zero temperature is paramount concern in cryobiology. susceptibility cryopreservation processes, especially freeze-thawing, stimulated considerable interest better understanding the mechanisms leading cell injury and inactivation. In this study, we assessed viability cold stress, through long-term supercooling experiments, versus freeze-thawing stress. Escherichia coli, Saccharomyces cerevisiae, leukemia were over time. Supercooled conditions maintained for 71 days -10 degrees C, 4 h -15 -20 without additives or emulsification. Results showed that could be inactivated by only action temperature, is, any water crystallization. loss upon exposure temperatures suggested caused shock which induced membrane damage. During holding time supercooled state, elevated permeability results uncontrolled mass transfer from thus leads a viability. With crystallization, shrink suddenly are exposed osmotic shock, induce abrupt frozen remain suspended residual unfrozen fraction liquid stress would cause damage However, severity such seems moderated type increased solute concentration suspension.

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