Proteomic analysis of common bean stem under drought stress using in-gel stable isotope labeling

摘要: Drought is an abiotic stress that strongly influences plant growth, development and productivity. Proteome changes in the stem of drought-tolerant common bean (Phaseolus vulgaris L.) cultivar Tiber have were when plants exposed to drought. Five-week-old subjected water deficit by withholding irrigation for 7, 12 17days, whereas control regularly irrigated. Relative content (RWC) leaves, as indicator degree cell tissue hydration, showed highest statistically significant differences between drought-stressed after 17days treatment, where RWC remained at 90% declined 45% stressed plants. Plants drought same developmental stage included quantitative proteomic analysis using in-gel stable isotope labeling proteins combination with mass spectrometry. The quantified grouped into several functional groups, mainly energy metabolism, photosynthesis, proteolysis, protein synthesis related defense stress. 70kDa heat shock greatest increase abundance under all proteins, suggesting its role protecting against re-establishing normal conformations thus cellular homeostasis. involved also increased stress, important recovery damaged cell's metabolic activities. Other this study proteolysis folding, which are necessary maintaining proper Taken together, these results reveal complexity pathways response stems enable comparison providing information further understand biochemical molecular mechanisms legume.