Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells

摘要: The restricted host-cell range and low titer of retroviral vectors limit their use for stable gene transfer in eukaryotic cells. To overcome these limitations, we have produced murine leukemia virus-derived which the envelope glycoprotein has been completely replaced by G vesicular stomatitis virus. Such can be concentrated ultracentrifugation to titers > 10(9) colony-forming units/ml infect cells, such as hamster fish cell lines, that are ordinarily resistant infection with containing protein. ability concentrate virus pseudotyped will facilitate therapy model studies other experiments require direct delivery vivo. availability also genetic nonmammalian species, including important zebrafish developmental system, through efficient introduction expression foreign genes.