Solid-state MAS NMR resonance assignment methods for proteins

摘要: The prerequisite to structural or functional studies of proteins by NMR is generally the assignment resonances. Since first solid-state MAS was conducted almost two decades ago, a wide variety different pulse sequences and methods have been proposed continue be developed. Traditionally, 2D 3D 13C-detected experiments used for backbone side-chain 13C 15N These found widespread use across field. But as hardware has changed higher spinning frequencies magnetic fields are becoming available, ability direct proton detection opening up new set based on triple-resonance experiments. This review describes using carbon at deuteration levels. isotopic labelling schemes an aid in difficult cases discussed well increasing number software packages that support manual automated resonance assignment.