Analysis of metabolites in plasma reveals distinct metabolic features between Dahl salt-sensitive rats and consomic SS.13BN rats

摘要: Salt-sensitive hypertension is a major risk factor for cardiovascular disorders. Our previous proteomic study revealed substantial differences in several proteins between Dahl salt-sensitive (SS) rats and salt-insensitive consomic SS.13(BN) rats. Subsequent experiments indicated role of fumarase insufficiency the development SS In present study, global metabolic profiling was performed using gas chromatography/mass spectrometry (GC/MS) plasma (n=9) (n=8) on 0.4% NaCl diet, designed to gain further insights into relationship alterations cellular intermediary metabolism predisposition hypertension. Principal component analysis data sets clear clustering separation profiles 23 differential metabolites were identified (P<0.05). Higher levels five TCA cycle metabolites, fumarate, cis-aconitate, isocitrate, citrate succinate, observed Pyruvate, which connects glycolysis, also increased Moreover, lower activity fumarase, aconitase, α-ketoglutarate dehydrogenase succinyl-CoA synthetase detected heart, liver or skeletal muscles The distinct features indicate abnormalities rats, may play predisposing developing