Lipoamide Dehydrogenase, Glutathione Reductase, Thioredoxin Reductase, and Thioredoxin A SIMULTANEOUS PURIFICATION AND CHARACTERIZATION OF THE FOUR PROTEINS FROM ESCHERICHIA COLI B
作者： Charles H. Williams , Giuliana Zanetti , L. David Arscott , Joan K. McAllister
DOI: 10.1016/S0021-9258(18)99415-9
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摘要: Abstract A procedure has been developed for the purification of lipoamide dehydrogenase, glutathione reductase, thioredoxin and from Escherichia coli B, in which four proteins are purified together initial stages separation effected two steps, individual purifications completed one or steps. The extinction coefficient FAD reductase determined. Amino acid analyses dehydrogenase given. half-cystine content E. is only one-half that found with pig heart enzyme.
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