Murine lupus monoclonal antibodies define five epitopes on two different Sm polypeptides.

摘要: Monoclonal anti-Sm (Smith) antibodies derived from the mouse strain MRL/lpr were isolated and characterized by binding to purified antigen, immunoprecipitating characteristic uridine-rich RNAs Hela cell extracts, Western blot analysis using rabbit thymus extract. Five different Sm epitopes demonstrated epitope blockade probing blots with monoclonal antibodies. Human serum inhibited each antibody indicating that both human bind same epitopes. bound 28,000 16,000 MW polypeptides, a small number also 14,000 polypeptide. The and/or polypeptide, provided evidence suggest these two polypeptides bear some structural similarities, but are distinct molecules.