Exercise preconditioning upregulates cerebral integrins and enhances cerebrovascular integrity in ischemic rats

摘要: We hypothesized that exercise preconditioning strengthens brain microvascular integrity against ischemia/reperfusion injury through the tumor necrosis factor (TNF)-integrin signaling pathway. Adult male Sprague Dawley rats (n = 24) were studied in: (1) (the animals run on a treadmill 30 min each day) for 3 weeks, (2) non-exercise. Six from group 12) subjected to stroke, remaining served as controls 6 × 2). Brain infarction and edema determined by Nissl staining. Cerebral integrin expression was detected immunochemistry stereological methods. In addition, we used flow cytometry address causal role of TNF-α in inducing integrins human umbilical vein endothelial cells under or vascular growth (VEGF) pretreatment. Exercise reduces stroke. Expressions subunit α1, α6, β1, β4 increased after exercise. reversed stroke-reduced expression. An vitro study revealed link between gradual upregulation (rather than VEGF) cellular integrins. These results demonstrated an increase cerebral decrease stroke preconditioning. The suggests during enhances neurovascular changes might be altered TNF-α.