Does removal of hydrogen change the electron energy-loss spectra of DNA bases?
作者： H. Schnabl
DOI: 10.1016/0304-3991(80)90020-0
关键词:
摘要: Quantum-chemical calculations using the CNDO/2 method are applied to radicals produced by cleaving hydrogen from DNA bases adenine, guanine, cytosine, and thymine. The resulting sequences of occupied molecular orbitals show that loss atom in most cases leads one phi electron. Consequently electron part energy-loss spectra will change considerably. In view this, it is possible due irradiation, as observed Isaacson et al., might be caused atoms. Such damage does not influence electron-microscopical image appreciably, long no deleterious secondary processes initiated.
elsevier.com
sci-hub.se 参考文章(11)
O. Merwitz, The cleavage of Tritium from solid, gamma-irradiated cytosine-5-T Radiat. Environ. Biophys., v. 11, no. 1, pp. 63-68. ,(1974) , 10.1007/BF01323101
C. Giessner-Prettre, A. Pullman, All valence-electrons calculations of the biological purines and pyrimidines Theoretical Chemistry Accounts. ,vol. 9, pp. 279- 287 ,(1968) , 10.1007/BF00529920
I. DIETRICH, H. FORMANEK, F. Fox, E. KNAPEK, R. WEYL, Reduction of radiation damage in an electron microscope with a superconducting lens system. Nature. ,vol. 277, pp. 380- 381 ,(1979) , 10.1038/277380A0
Adolf Müller, The formation of radicals in nucleic acids, nucleoproteins, and their constituents by ionizing radiations. Progress in Biophysics & Molecular Biology. ,vol. 17, pp. 99- 147 ,(1967) , 10.1016/0079-6107(67)90005-3
Herbert Schnabl, An Interpretation of Radiation Damage Measurements in DNA Bases Radiation Research. ,vol. 67, pp. 184- 186 ,(1976) , 10.2307/3574510
M. Isaacson, D. Johnson, A. V. Crewe, Electron Beam Excitation and Damage of Biological Molecules; Its Implications for Specimen Damage in Electron Microscopy Radiation Research. ,vol. 55, pp. 205- ,(1973) , 10.2307/3573678
J. A. Pople, G. A. Segal, Approximate Self-Consistent Molecular Orbital Theory. II. Calculations with Complete Neglect of Differential Overlap Journal of Chemical Physics. ,vol. 43, ,(1965) , 10.1063/1.1701476
M. A. Hayat, Principles and Techniques of Electron Microscopy: Biological Applications ,(1976)
Jerry M. Rice, Gerald O. Dudek, Michael Barber, Mass Spectra of Nucleic Acid Derivatives. Pyrimidines Journal of the American Chemical Society. ,vol. 87, pp. 4569- 4576 ,(1965) , 10.1021/JA00948A029
Michael Isaacson, Interaction of 25 keV Electrons with the Nucleic Acid Bases, Adenine, Thymine, and Uracil. I. Outer Shell Excitation The Journal of Chemical Physics. ,vol. 56, pp. 1803- 1812 ,(1972) , 10.1063/1.1677456