Identification of HSP70 gene in Corythucha ciliata and its expression profiles under laboratory and field thermal conditions.

摘要: Previous laboratory studies have demonstrated that insects can tolerate high temperatures by expressing inducible heat shock proteins (HSPs). This HSP-based tolerance, however, has seldom been studied under field conditions. Here, we cloned the HSP70 gene of Corythucha ciliata (Cchsp70), an invasive insect species with substantial thermal tolerance in subtropical China. We also compared relative mRNA expression levels Cchsp70 response to controlled temperature treatments (2 h at 33–43 °C 2 intervals laboratory) and natural increases (08:00–14:00 2-h intervals, 29.7–37.2 °C) on a hot summer day field. The complete cDNA is 2256 bp long 1917 open reading frame encodes protein (CcHSP70) 639 amino acids. significantly increased both At similar temperatures, were much higher than laboratory. These results suggest CcHSP70 contributes C. factors addition stress may induce