Biosynthesis of peptidoglycan in Gaffkya homari: processing of nascent glycan by reactivated membranes.

摘要: Membranes from Gaffkya homari reactivated by freezing and thawing were used to study the processing events involved in assembly of both sodium dodecyl sulfate (SDS)-insoluble peptidoglycan (PG) SDS-soluble PG. The ability reactivate membranes for synthesis these polymers provided an opportunity monitor those that are not influenced wall-linked In G. homari, formation cross-links requires selective actions DD-carboxypeptidase, LD-carboxypeptidase, NE-(DAla)-Lys transpeptidase. Time courses cross-link formation, as measured amounts amidated bisdisaccharide peptide dimer nonamidated dimer, showed a lack correlation with SDS-insoluble PG, which is significantly cross-linked when synthesized absence penicillin G, was precursor presence un-cross-linked PG synthesized. This also utilized processed after removal beta-lactam. protocol method separating stages elongation processing. Cross-linkage various fractions ranged 0 19% 2 24% Thus, results indicated there no direct between SDS insolubility degree cross-linkage. Instead, they suggested additional features may contribute SDS.