Rate of false positive reactions in 11 M. gallisepticum and M. synoviae serological tests in samples obtained from SPF birds inoculated with heterologous mycoplasma species.

摘要: No recent information is available on the specificity of current M. synoviae (Ms) and gallisepticum (Mg) serological tests. In this study performance a currently Mg Ms Rapid Plate Agglutination (RPA) test, three Mg, Mg/Ms combination ELISAs were evaluated SPF sera that obtained from days (D) 0-28 after gallinarum, imitans or gallinaceum inoculation, sham inoculation without inoculation. Tracheal swabs for mycoplasma culture before (D0), 7 28 post (d.p.i.) in all groups except inoculated group. The different species colonized well. early stage (7-14 d.p.i.) with heterologous species, varied 85% to 100% RPA test 70% test. both was samples ruled out effect medium. late (21-28 seven two where 95% found However, not significantly other tests these groups. These results show it advisable establish seromonitoring programmes alone as frequently occur poultry.