In vitro selection of nucleoprotein enzymes

摘要: Natural nucleic acids frequently rely on proteins for stabilization or catalytic activity. In contrast, selected in vitro can catalyze a wide range of reactions even the absence proteins. To augment with protein functionalities, we have developed technique selection protein-dependent ribozyme ligases. After randomizing previously ligase, L1, variants that required one two cofactors, tyrosyl transfer RNA (tRNA) synthetase (Cyt18) hen egg white lysozyme. The resulting nucleoprotein enzymes were activated several thousand fold by their cognate effectors, and could specifically recognize structures native Protein-dependent ribozymes potentially be adapted to novel assays detecting target proteins, method's generality may allow high-throughput identification capable recognizing sizable fraction proteome.