Methylxanthines Inhibit Primary Amine Oxidase and Monoamine Oxidase Activities of Human Adipose Tissue.

摘要: Background: Methylxanthines including caffeine and theobromine are widely consumed compounds were recently shown to interact with bovine copper-containing amine oxidase. To the best of our knowledge, no direct demonstration any interplay between these phytochemicals human primary oxidase (PrAO) has been reported date. We took advantage coexistence PrAO monoamine (MAO) activities in subcutaneous adipose tissue (hScAT) test interaction several methylxanthines enzymes, which involved many key pathophysiological processes. Methods: Benzylamine, methylamine, tyramine used as substrates for MAO homogenates depots obtained from overweight women undergoing plastic surgery. tested or inhibitors by fluorimetric determination hydrogen peroxide, an end-product oxidation. Results: Semicarbazide-sensitive activity was inhibited theobromine, caffeine, isobutylmethylxanthine (IBMX) while theophylline, paraxanthine, 7-methylxanthine had little effect. Theobromine 54% at 2.5 mM. Overall, relationship methylxanthine structure degree inhibition similar that seen PrAO, although higher concentrations (mM) required inhibition. also oxidation MAO, limits its solubility a DMSO vehicle. At doses than 12 % v/v, impaired activity. millimolar IBMX, other lesser extent. Conclusions: This preclinical study extrapolates previous findings tissues. Given is potential target anti-inflammatory drugs, it indicates alongside phosphodiesterase adenosine receptor antagonism, could contribute health benefits methylxanthines, especially their effects.