A rapid radioimmunoassay for plasma estrogen

摘要: Abstract The plasma estradiol equivalent is the total estrogen quantitated by a rapid radioimmunoassay on unextracted plasma. For radioimmunoassay, 0.1 or 0.2 ml. of precipitated with methanol, and supernate utilized. An internal standard not required to correct for procedural losses. Dextran-coated characoal solution used separate free bound steroid. assay sensitivity 5 pg. per tube (35 milliliter sample). coefficient variation at this level 14.3 cent. correlation estrone, estradiol, estriol good (r = 0.962). Thirty samples can be analyzed reported within 8 hours.