Direct, simplified, and sensitive assay of angiotensin II in plasma extracts performed with a high-affinity monoclonal antibody.
作者： D Simon , B Romestand , H Huang , G Badouaille , J A Fehrentz
DOI: 10.1093/CLINCHEM/38.10.1963
关键词:
摘要: A very simple, fast, and sensitive RIA of angiotensin (Ang) II has been developed, based on a monoclonal antibody with high affinity specificity, making possible the direct measurement circulating Ang in human plasma after solid-phase extraction. The purified 4D8 an association constant 1.3 x 10(11) L/mol cross-reactivity < 1% for I. assay can detect as little 0.8 fmol 2 mL is not influenced by presence Analytical recoveries between 112% 116% were obtained added to at physiological concentrations. Comparison reversed-phase, high-performance liquid chromatographic method followed measure samples from normal hypertensive subjects--and normotensive subjects before acute inhibition angiotensin-converting enzyme captopril (50 mg)--showed degree correlation (r2 = 0.93) two methods.
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