Day/night rhythms in gene expression of the normal murine heart.

摘要: Molecular circadian oscillators have recently been identified in heart and many other peripheral organs; however, little is known about the physiologic significance of gene cycling periphery. While general temporal profiles expression described under constant lighting conditions, patterns normal day/night conditions may be distinctly different. To understand how contributes to cardiac function, especially human beings, it crucial examine these 24-h light dark environments. High-density oligonucleotide microarrays were used assess myocardial 12,488 murine genes at 3-h intervals cycling. Variation genetic activity was considerable, as 1,634 (~13% analyzed) exhibited statistically significant changes across cycle. Some rhythmic expression, others showed abrupt change light-to-dark dark-to-light transitions. Importantly, that rhythms mapped key biological pathways, including for example cellular growth remodeling, well transcription, translation, mitochondrial respiration, signaling pathways. Gene remarkably different day versus night. driven by central pacemaker, while appear responses dark. This has important implications regarding our understanding molecular physiology controlled, organ growth, renewal, disease, comparative most appropriate times administration therapy.