Expression of the thyroid sodium/iodide symporter in Xenopus laevis oocytes.
作者： F Vilijn , N Carrasco
DOI: 10.1016/S0021-9258(18)80151-X
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摘要: Abstract Poly(A+) RNA isolated from FRTL-5 cells (a continuous line of cultured and fully functional rat thyroid (Ambesi-Impiombato, F. S., Parks, L. A. M., Coons, H. G. (1980) Proc. Natl. Acad. Sci. U. S. 77, 3455-3459] was injected into Xenopus laevis oocytes, the expression Na+/I- symporter in plasma membrane assayed by measuring Na+-dependent ClO4--sensitive uptake 125I. Expression detected as a 7-fold average increase transport over background, 5-6 days after injection. subsequently fractionated sucrose gradient centrifugation, fractions were for their ability to induce I- activity. The poly(A+) encoding found fraction containing messages 2.8-4.0 kilobases length.
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