Bovine intra-mammary challenge with Streptococcus dysgalactiae spp. Dysgalactiae to explore the effect on the response of Complement activity.

摘要: Recently published work as described by the authors highlighted extent of Complement activity in bovine milk. Localised mastitis infection occurring mammary glands dairy cows is readily detectable levels somatic cells Thus, it opportune to monitor milks association with animal's innate immune response infection. Preliminary screening milk samples taken randomly showed that a high cell count (SCC) reduced growth Complement-sensitive strain E. coli O111 greater (P < 0·05) than when marker microorganism was grown heated for purpose inactivating Complement. A follow-up study set out determine effect on sub-clinical induced gland four lactating cows. The Str. dysgalactiae spp. inoculation into selected individual udder quarters each animal followed monitoring SCC from segregated during subsequent milking. At 72 and 96 h post (PI), SCCs challenged quarter were increased compared normal values. same time, bactericidal sequestration assay identified inhibition can be directly linked those affected inflammation. more effective limiting growth. Milks unchallenged all significantly less at reducing 0·05). An ELISA targeting specific activation components pathways confirmed bacterial observed attributable higher quarters, i.e., SCC. self-limiting three animals their returned within 14 d PI, while fourth cow required brief antibiotic intervention.