EFECTO DEL USO DEL SUELO SOBRE COMUNIDADES MICROBIANAS DE SISTEMAS DE PASTOREO Y CULTIVOS DE CAÑA DE AZUCAR DEL VALLE DEL CAUCA
作者： Johan Sebastián , Sáenz Medina
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摘要: The different uses that are given to soils affect their physicochemical properties, which may have an effect on microbial communities. To determine these effects it must be understood how the methods used in ecology studies estimation of diversity. In present study was analyzed: 1) ADN extraction (PowerSoil Kit vs Griffiths method) and purification method (removing contaminants) 2) land use a conventional or agro-ecological way, grazing systems sugar cane crops two regions Valle del Cauca. communities were studied by sequencing V4 region 16S rRNA gene Next Generation Sequencing (MiSeq, Illumina).
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