A survey of genes in Eimeria tenella merozoites by EST sequencing.

摘要: A study of about 500 expressed sequence tags (ESTs), derived from a merozoite cDNA library, was initiated as an approach to generate larger pool gene information on Eimeria tenella. Of the ESTs, 47.7% had matches with entries in databases, including ribosomal proteins, metabolic enzymes and proteins other functions, which 14.3% represented previously known E. tenella genes. Thus over 50% ESTs no significant database matches. The EST dataset contained range highly abundant genes comparable that found T. gondii may thus reflect importance such molecules biology apicomplexan organisms. However, comparison two datasets revealed very few homologies between sequences apical organelle molecules, provides evidence for divergence these closely-related parasites. data presented underpin potential value strategy discovery novel allow more rapid increase knowledge understanding expression life cycle stage spp.