Effect of pyruvate on oxidant injury to isolated and cellular DNA.

摘要: Effect of pyruvate on oxidant injury to isolated and cellular DNA. Drawing upon the capacity detoxify H 2 O , we demonstrate that (i) protects against -dependent, hydroxyl radical-mediated degradation DNA; (ii) reduces amount 8-hydroxy-2-deoxyguanosine detected following oxidative DNA (iii) diminishes amounts detectable radical generated by a -dependent system. Compared mannitol, weakly induced -independent, radical-generating The protective effects -instigated damage were also evinced in cells culture exposed . In contrast its DNA, failed offer convincing protection another intracellular, -vulnerable target, glyceraldehyde-3-phosphate dehydrogenase. conferred intracellular targets is thus influenced nature target Pyruvate was markedly model cytotoxicity concomitant depletion glutathione inhibition catalase activity; can function as an antioxidant this latter model, no evidence observed. Pyruvate, catalase, potent protector organic peroxides, finding cannot be explained scavenging differences content or attenuation We conclude while key pathogenetic event stress such nuclear changes may not universally subserve critical role models cell death. capabilities extend beyond include peroxides.